Plate Mapping

Alto and Stratus plate readers have 96 discrete detectors centered in standard 96-well plates. Reading other plate types is possible, given that the plate being measured has sufficient overlap with the 96 discrete filters in the stratus. The detectors used for 6-well, 12-well and Cerillo Co-culture plates are displayed below:

6-Well Plate Detector Mapping

6-well detectors used

12-Well Plate Detector Mapping

12-well detectors used

Co-culture Plate Detector Mapping

co-culture detectors used

24-Well Plate Detector Mapping

Stratus devices are capable of measuring 24-well plates with the following mapping. However, note that there is no standard spacing for 24-well plates, so your results may vary. Additionally, because the detectors used for some wells are not very close to the center, those results may have systematic error. 24-well detectors used 24-well plate templates can be enabled on any device by navigating to the application settings and typing ‘24well’. A checkbox will appear to enable/disable 24-well plate templates. Be advised that the use of 24-well plates in Cerillo plate readers is not recommended because of well alignment.

Remapping default readings to different plate types

Experiments started with a button press or with the default plate type can be remapped after they have finished from the plate layout dialog. This process will take the detectors for each well in the new plate type, average them, and then show the new plate type for that experiment. The default plate type for both the Alto and Stratus devices is 96-well plate.

Important

Remapping experiments to different plate types only changes the experiment on your computer, it does not make modifications to the data on the device. So if you move the device to a different computer, the original plate type may still show up.