Standard Curve

The Standard Curve tab is available for endpoint experiments with standard curve information entered. See how to enter standard curve info here. Endpoint Graph Results

Number

Element

Description

1

Group Visibility Toggle

Click the checkbox for a group to toggle its visibility in the graph. Wells not assigned to a group can be viewed with the UNGROUPED toggle.

2

Select All

Toggle all groups to be visible on the graph.

3

Select None

Toggle off visibility of all groups.

4

View Select

Toggle between Group Average of the wells assigned to a group or Individual Wells to see each well plotted

5

Export Graph

Export the graph as a .png or .svg file for lab meetings or presentations. .png export size can be selected prior to export. .svg images are vector-based so they scale automatically.

6

Standard Curve Graph

Measured samples are the samples with known concentrations entered in the Endpoint Plate Layout and their measured optical density. Those samples are used to calculate a standard curve using the model selected in the Fit Select. Unknown or blank groups are added to the graph with the concentration calculated from the equation for the standard curve.

7

Fit Select

Choose between Linear Regression and Four-Parameter Logistic Regression (4PL) as the model to fit the measured samples to an equation. This selection should persist the next time the experiment is opened on the same computer.

8

Equation Visibility Toggle

Click to show or hide the equation generated to fit the data. The R2 value denotes how well the curve fits the data with a value close to 1 being a good fit.